Trail Location: South
- A. D. Barnes Park
- Amberjack Environmental Park
- Arlington Park
- Arthur R. Marshall Loxahatchee National Wildlife Refuge: Headquarters Area
- Bahia Honda State Park
- Big Cypress National Preserve
- Bill Baggs Cape Florida State Park
- Biscayne National Park: Convoy Point
- Blind Pass Beach Park
- Bowditch Point Regional Park
- Brian Piccolo Park
- Caloosahatchee Regional Park
- Caspersen Beach
- Castellow Hammock Preserve
- Cayo Costa State Park
- Celery Fields
- Charlotte Flatwoods Environmental Park
- Charlotte Harbor Environmental Center: Alligator Creek Preserve
- Charlotte Harbor Environmental Center: Cedar Point Environmental Park
- Charlotte Harbor Preserve State Park: Little Pine Island Trail
- Charlotte Harbor Preserve State Park: North Cape Flats Trail
- Charlotte Harbor Preserve State Park: Old Datsun Trail
- City of West Palm Beach Grassy Waters Preserve
- Collier-Seminole State Park
- Conservancy of Southwest Florida Nature Center
- Corkscrew Swamp Sanctuary
- Crandon Park: Bear Cut Preserve and Beach
- Crane Point Museum and Nature Center
- CREW: Corkscrew Marsh Unit
- Crowley Museum and Nature Center
- Curry Hammock State Park
- Dagny Johnson Key Largo Hammock Botanical State Park
- Deering Estate at Cutler
- Delnor-Wiggins Pass State Park
- Delray Oaks Natural Area
- Dinner Island Ranch Wildlife Management Area
- Dry Tortugas National Park
- Eagle Lakes Community Park
- Easterlin Park
- Estero Bay Preserve State Park
- Everglades and Francis S. Taylor Wildlife Management Area
- Everglades National Park: Gulf Coast Visitor Center
- Everglades National Park: Main Entrance
- Everglades National Park: Shark Valley Visitor Center
- Fakahatchee Strand Preserve State Park
- Fern Forest Nature Center
- Fisheating Creek Wildlife Management Area: East Entrance
- Fisheating Creek Wildlife Management Area: West Entrance
- Fort Zachary Taylor Historic State Park
- Fred C. Babcock/Cecil M. Webb Wildlife Management Area
